X-Received: by 2002:a05:622a:800f:b0:41c:2d56:bbf5 with SMTP id jr15-20020a05622a800f00b0041c2d56bbf5mr22181qtb.11.1701788743469; Tue, 05 Dec 2023 07:05:43 -0800 (PST) X-Received: by 2002:a05:6808:2122:b0:3b9:b430:2908 with SMTP id r34-20020a056808212200b003b9b4302908mr2515048oiw.11.1701788742793; Tue, 05 Dec 2023 07:05:42 -0800 (PST) Path: csiph.com!1.us.feeder.erje.net!feeder.erje.net!usenet.blueworldhosting.com!diablo1.usenet.blueworldhosting.com!peer03.iad!feed-me.highwinds-media.com!news.highwinds-media.com!news-out.google.com!nntp.google.com!postnews.google.com!google-groups.googlegroups.com!not-for-mail Newsgroups: comp.databases.btrieve Date: Tue, 5 Dec 2023 07:05:42 -0800 (PST) Injection-Info: google-groups.googlegroups.com; posting-host=205.185.222.148; posting-account=GEgLJgoAAABvborjf-60Dkf53cr_PXGz NNTP-Posting-Host: 205.185.222.148 User-Agent: G2/1.0 MIME-Version: 1.0 Message-ID: <3eb63c34-4bbd-4127-aef7-1b4874cee765n@googlegroups.com> Subject: Dg Foto Art V5.2 Classic Gold V2.0 With Templates.15 From: Velma Kuhnert Injection-Date: Tue, 05 Dec 2023 15:05:43 +0000 Content-Type: text/plain; charset="UTF-8" Content-Transfer-Encoding: quoted-printable X-Received-Bytes: 2976 Xref: csiph.com comp.databases.btrieve:73 Background and aims Proteome-based biomarker studies are targeting proteins= that could serve as diagnostic, prognosis, and prediction molecules. In th= e clinical routine, immunoassays are currently used for the absolute quanti= fication of such biomarkers, with the major limitation that only one molecu= le can be targeted per assay. The aim of our study was to test a mass spect= rometry based absolute quantification method for the verification of plasma= protein sets which might serve as reliable biomarker panels for the clinic= al practice. Methods Six EDTA plasma samples were analyzed after tryptic di= gestion using a high throughput data independent acquisition nano-LC Q-TOF = UDMSE proteomics approach. Synthetic Escherichia coli standard peptides wer= e spiked in each sample for the absolute quantification. Data analysis was = performed using ProgenesisQI v2.0 software (Waters Corporation). Results Ou= r method ensured absolute quantification of 242 non redundant plasma protei= ns in a single run analysis. The dynamic range covered was 105. 86% were re= presented by classical plasma proteins. The overall median coefficient of v= ariation was 0.36, while a set of 63 proteins was found to be highly stable= . Absolute protein concentrations strongly correlated with values reviewed = in the literature. Conclusions Nano-LC Q-TOF UDMSE proteomic analysis can b= e used for a simple and rapid determination of absolute amounts of plasma p= roteins. A large number of plasma proteins could be analyzed, while a wide = dynamic range was covered with low coefficient of variation at protein leve= l. The method proved to be a reliable tool for the quantification of protei= n panel for biomarker verification in the clinical practice. PMID:29151793 Dg Foto Art V5.2 Classic Gold V2.0 With Templates.15 Download File https://t.co/cdxbmqnXzV eebf2c3492